Overview

This lab, Under the supervision of Professor Makoto Kobayashi, is currently focusing on molecular mechanisms regulating defense against a variety of stresses, such as electrophilic stress, oxidative stress, ER stress, and so on. The other major focus in the lab is how cell fate decisions are made, especially in the case of hematopoietic lineages.

We are using zebrafish as a model organism that has been shown to be useful for developmental, genetic, toxicological, physiological and imaging studies. The zebrafish offers several advantages in molecular biological studies, e.g., easy knockdown of individual genes, clear in vivo visualization of fluorescent proteins and imaging probes, rapid generation of stable transgenic lines.

In this course you will learn about methods for the gene knock-down analysis using zebrafish and CRISPR-Cas9.

English Task

Please download the PDF document here. Print it out. There are three parts. Please do the first part before watching the video below, the second part is to be done during the video, and the third part after finishing the video and task. In the first part it asks you to write Japanese translations of English words, but if you are not Japanese, please do this in your first language. Upon completion of the exercise, please scan/photograph your work and email to: mayers@md.tsukuba.ac.jp (Thomas Mayers)

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Gene Knock-down analysis using zebrafish and CRISPR-Cas9

Watch the video

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Your Task

Please download the Word document here, carefully read the questions and write your responses. You can re-watch the videos as many times as you like. You can do extra research by downloading the research papers below and searching for information on the Internet. Responses must be typed. Upon completion of the exercise, please email to: mayers@md.tsukuba.ac.jp (Thomas Mayers)

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